Periplasmic Oligopeptide-Binding Protein

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASN-246   ASN-247  7.0 7.2 12.7 -10.1 71.0 79.5 65.2  ASN-247   MET-248  6.2 6.7 -2.5 0.1 168.3 170.4 -21.2  MET-248   PRO-249  4.9 5.5 13.6 -16.5 59.2 56.8 13.0  PRO-249   ILE-250  5.9 7.0 10.7 -16.8 67.5 59.0 17.6  ILE-250   GLU-251  4.1 6.1 20.0 -0.7 16.9 11.6 72.8  GLU-251   LEU-252  4.4 7.0 18.0 -61.1 77.9 71.3 -48.2  LEU-252   PHE-253  4.3 5.0 31.9 -15.9 105.0 114.4 -72.7  PHE-253   GLN-254  2.3 2.4 5.2 -1.2 53.1 56.8 -17.0  GLN-254   LYS-255  1.7 1.0 -0.4 -5.0 134.4 128.4 -0.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-268   TYR-269  6.8 6.6 -2.4 -10.9 57.8 58.1 6.0  TYR-269   LEU-270  3.7 3.4 -10.4 -10.2 43.6 50.7 46.2  LEU-270   CYS-271  2.5 2.4 6.2 0.1 85.2 92.7 37.3  CYS-271   THR-272  2.4 2.5 5.6 -6.1 67.5 65.9 -4.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TYR-484   TYR-485  6.6 6.6 0.8 -0.5 132.3 130.4 16.0  TYR-485   VAL-486  3.2 3.1 3.1 -5.9 99.1 100.1 24.5  VAL-486   ASN-487  3.5 2.7 7.3 -5.8 92.4 91.5 28.4  ASN-487   ALA-488  4.0 3.4 -2.5 -2.4 29.6 26.4 23.4  ALA-488   ARG-489  4.8 4.1 -0.9 -2.1 77.6 79.7 -45.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees