DNA Polymerase IV (Family Y)

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-34   GLY-35  29.1 29.3 -15.5 16.3 21.9 18.3 -55.1  GLY-35   ARG-36  30.2 30.2 -15.9 14.0 78.9 74.5 32.6  ARG-36   PHE-37  33.6 33.6 -16.9 15.7 60.4 59.1 69.9  PHE-37   GLU-38  35.6 35.6 6.8 -5.4 82.0 82.3 119.4  GLU-38   ASP-39  35.7 35.9 5.0 -6.1 163.2 164.9 19.0  GLU-38   ASP-39  35.7 35.9 5.0 -6.1 163.2 164.9 19.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-38   ASP-39  35.7 35.9 5.0 -6.1 163.2 164.9 19.0  GLU-38   ASP-39  35.7 35.9 5.0 -6.1 163.2 164.9 19.0  ASP-39   SER-40  34.3 34.6 10.5 -14.4 135.2 133.7 139.8  SER-40   GLY-41  34.0 34.2 7.0 -10.1 116.4 116.5 131.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ARG-238   THR-239  16.7 16.9 -8.3 -1.1 138.3 140.6 -318.8  ARG-238   THR-239  5.2 4.8 -8.3 -1.1 7.4 9.2 491.0  THR-239   ARG-240  4.2 4.0 0.5 -1.9 110.9 109.8 -284.4  ARG-238   THR-239  16.7 16.9 -8.3 -1.1 138.3 140.6 -318.8  ARG-238   THR-239  5.2 4.8 -8.3 -1.1 7.4 9.2 491.0  THR-239   ARG-240  4.2 4.0 0.5 -1.9 110.9 109.8 -284.4  ARG-240   VAL-241  7.4 7.2 -9.8 16.4 33.3 29.6 -261.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees