Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-85   GLY-86  5.4 5.8 -3.9 -14.5 60.6 62.6 32.2  GLY-86   PRO-87  2.4 2.9 21.6 -12.1 92.5 109.2 -5.3  PRO-87   GLU-88  4.9 5.3 -77.0 36.1 38.5 40.2 127.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-186   PRO-187  9.4 9.5 -13.6 11.2 55.5 65.7 1.9  PRO-187   ALA-188  8.0 7.9 -13.8 -3.5 101.8 109.8 -7.3  ALA-188   VAL-189  5.7 5.3 18.5 -5.8 113.3 133.5 -0.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees