Rho Guanine Nucleotide Exchange Factor 12

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLN-976   ALA-977  10.5 10.5 -4.2 -14.3 68.7 75.3 3.0  ALA-977   VAL-978  9.0 8.7 7.4 12.6 44.8 33.2 16.4  VAL-978   LYS-979  7.8 8.3 -26.3 27.6 100.3 105.5 8.3  LYS-979   GLU-980  6.3 6.5 -7.8 10.0 55.9 55.1 -7.4  GLU-980   ALA-981  4.3 4.3 1.9 -8.2 75.9 70.7 -16.1  ALA-981   GLU-982  3.7 4.0 -15.0 44.0 139.7 154.0 64.2  GLU-982   ASN-983  1.8 2.9 -5.7 -8.9 84.1 83.6 -3.2  ASN-983   LYS-984  2.2 1.1 -7.5 -8.6 53.9 67.2 0.9  LYS-984   GLN-985  1.9 2.3 21.4 -9.7 50.0 50.7 18.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees