Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLU-4   MET-5  12.5 11.4 -4.7 -14.2 60.6 56.3 76.3
 MET-5   PRO-6  8.8 8.3 19.5 -9.8 90.3 75.2 -10.9
 PRO-6   GLN-7  9.0 9.0 5.4 11.7 53.5 57.2 52.5
 GLN-7   PRO-8  8.4 8.4 -1.4 -17.9 87.9 83.3 -16.3
 PRO-8   LYS-9  10.4 10.7 18.9 13.3 29.8 29.0 178.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-17   PRO-18  11.6 11.5 -6.8 0.5 109.8 110.3 7.3
 PRO-18   LEU-19  15.1 15.1 -1.4 22.6 175.4 169.0 120.2
 LEU-19   LEU-20  14.0 14.3 -24.2 30.1 111.8 115.4 -11.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLU-38   ILE-39  3.7 3.7 -0.8 0.0 154.6 153.5 -11.8
 ILE-39   PHE-40  3.6 3.3 3.2 0.8 48.9 47.6 38.1
 PHE-40   LYS-41  4.3 4.1 8.0 -8.2 35.2 35.8 17.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLY-46   ARG-47  5.1 4.4 39.1 10.8 41.1 35.7 238.3
 ARG-47   VAL-48  3.9 4.4 -9.7 -0.5 136.1 134.2 -109.5
 VAL-48   THR-49  1.4 2.0 -2.1 18.7 140.3 144.9 101.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees