Manganese-Dependent Inorganic Pyrophosphatase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-185   LYS-186  6.3 6.2 -5.6 -7.4 63.5 64.3 11.7  LYS-186   ALA-187  4.3 4.0 13.4 -3.7 147.6 154.7 -9.0  ALA-187   GLY-188  4.4 4.2 -22.1 -22.4 98.3 96.9 -12.1  GLY-188   ALA-189  4.2 4.5 62.7 -4.2 107.3 67.4 10.7  ALA-189   ASP-190  2.4 1.6 69.5 -169.2 122.6 131.7 82.5  ASP-190   LEU-191  1.5 1.4 -2.0 1.2 93.0 71.9 11.6  LEU-191   SER-192  4.9 5.2 26.0 -0.2 118.7 140.0 -21.7  SER-192   LYS-193  7.0 6.3 -51.0 34.4 20.9 42.9 12.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees