Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PHE-173   ILE-174  2.1 1.2 -6.2 3.9 140.7 144.6 3.4
 ILE-174   THR-175  1.2 1.3 1.3 12.0 51.6 50.6 17.4
 THR-175   SER-176  2.7 2.1 -14.6 2.1 68.3 75.0 30.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASN-213   ASP-214  4.9 5.1 12.7 -5.9 57.9 55.6 18.5
 ASP-214   LEU-215  4.6 4.6 -10.6 9.2 91.0 99.7 14.6
 LEU-215   VAL-216  1.9 1.5 3.5 4.6 49.2 47.1 17.9
 VAL-216   ASP-217  1.4 1.9 5.9 -16.6 20.4 21.0 -35.3
 ASP-217   LYS-218  1.7 1.9 7.3 11.7 75.0 77.5 43.1
 LYS-218   ILE-219  3.2 3.0 -11.6 5.2 97.3 107.4 7.0
 ILE-219   ILE-220  4.0 3.6 -3.3 -0.5 156.4 159.6 -24.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees