Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-86   PRO-87  2.9 2.5 29.4 -22.9 84.7 94.2 -6.6  PRO-87   GLU-88  3.0 3.3 -72.6 32.8 42.1 44.8 134.0  GLU-88   ILE-89  6.2 6.2 11.7 -8.3 90.7 78.1 -46.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-177   ASN-178  10.9 11.3 -29.6 17.0 106.1 104.7 1.4  ASN-178   LEU-179  8.3 8.7 -23.3 15.3 113.3 107.6 -13.1  LEU-179   PRO-180  9.2 9.7 -5.3 17.6 131.9 124.2 -26.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-181   CYS-182  10.1 9.2 25.1 -15.6 32.8 46.2 -23.0  CYS-182   ASP-183  10.7 10.8 -0.6 -0.4 126.1 137.6 -4.9  ASP-183   VAL-184  10.3 10.7 -20.2 10.5 152.6 150.7 -50.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-186   PRO-187  7.2 7.2 -1.0 2.0 66.3 74.0 -8.8  PRO-187   ALA-188  6.1 5.3 -30.1 14.8 110.7 113.6 -39.8  ALA-188   VAL-189  4.8 3.9 -12.0 8.0 64.0 52.1 28.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees