Fusion Protein Of Maltose-Binding Periplasmic Protein And Parathyroid Hormone/parathyroid Hormone-Related Peptide Receptor

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS--174   TYR--173  7.7 7.9 10.6 -14.9 150.7 150.6 -34.8  TYR--173   GLU--172  7.1 7.3 0.7 0.1 103.0 107.6 -7.2  GLU--172   ASN--171  7.1 7.5 4.6 -2.0 127.3 127.8 -18.1  ASN--171   GLY--170  6.7 7.1 1.8 -4.1 119.6 119.7 11.8  GLY--170   LYS--169  4.2 4.7 0.9 -1.3 60.8 61.7 -93.1  LYS--169   TYR--168  2.4 2.8 3.5 1.5 27.4 21.5 116.3  TYR--168   ASP--167  3.5 3.5 -3.8 5.0 126.5 128.1 -15.0  ASP--167   ILE--166  2.5 2.8 -0.9 4.3 27.6 30.4 -27.8  ASP--167   ILE--166  2.5 2.8 -0.9 4.3 27.6 30.4 -27.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP--167   ILE--166  2.5 2.8 -0.9 4.3 27.6 30.4 -27.8  ASP--167   ILE--166  2.5 2.8 -0.9 4.3 27.6 30.4 -27.8  ILE--166   LYS--165  1.0 0.9 -6.6 3.3 34.6 31.6 16.7  LYS--165   ASP--164  2.5 2.5 -3.3 4.8 73.4 73.6 51.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASN-23   ALA-24  5.3 5.4 -4.9 0.5 11.8 14.1 63.9  ALA-24   ALA-25  5.5 5.4 -2.4 4.1 72.1 74.9 8.1  ALA-25   ALA-26  1.8 1.7 3.2 -10.6 73.3 72.3 14.0  ALA-26   GLU-27  4.2 4.1 8.2 -3.5 132.5 128.8 -44.2  GLU-27   PHE-28  6.7 6.6 3.3 -9.4 149.7 146.8 45.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees