Restriction Endonuclease EcorII

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-165   GLY-166  11.5 12.2 17.7 -23.9 81.8 100.1 0.2  GLY-166   GLN-167  7.8 8.8 41.3 -31.5 22.2 42.6 28.7  GLN-167   ILE-168  7.4 8.1 6.6 -13.4 50.9 42.0 -26.1  ILE-168   LEU-169  9.6 9.0 -6.0 10.1 82.0 75.7 17.3  LEU-169   GLY-170  8.9 8.6 165.0 -168.2 95.9 109.9 -5.8  GLY-170   GLY-171  5.1 5.6 18.9 -20.7 53.7 59.7 -28.8  GLY-171   LEU-172  5.3 6.0 29.3 -2.8 29.2 29.4 85.7  LEU-172   SER-173  3.1 3.5 5.4 8.5 86.9 55.9 45.0  SER-173   LEU-174  5.8 4.9 -25.1 -9.5 104.4 105.2 -28.8  LEU-174   GLN-175  7.3 6.2 -34.2 129.7 141.9 140.1 278.0  GLN-175   GLN-176  10.1 8.4 47.8 27.7 27.3 89.5 61.8  GLN-176   ALA-177  10.2 11.6 -109.7 55.0 113.7 149.7 -158.2  ALA-177   PRO-178  13.2 12.0 -129.4 -15.0 169.5 95.4 -447.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-234   LEU-235  7.3 8.2 -3.4 -2.8 104.7 108.6 -8.8  LEU-235   HIS-236  9.6 10.0 28.3 -18.8 119.6 120.0 -4.8  HIS-236   VAL-237  9.3 9.4 -2.7 -40.5 3.0 17.1 170.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-255   ASN-256  5.1 4.3 -5.1 6.9 74.9 74.2 -12.7  ASN-256   SER-257  6.9 5.3 -7.3 -5.5 16.5 10.9 50.4  SER-257   VAL-258  8.2 6.4 2.5 0.3 125.0 122.0 -17.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees