Arginine Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-288   LYS-289  18.5 18.5 -17.6 -4.0 54.6 30.2 31.4  LYS-289   LEU-290  19.7 19.1 -4.9 -2.5 23.3 37.2 22.7  LEU-290   ALA-291  17.1 16.0 0.8 -43.7 116.1 101.4 26.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  HIS-315   THR-316  25.2 19.8 72.2 -61.8 149.7 129.0 0.4  THR-316   GLU-317  25.8 20.4 177.4 23.1 99.2 82.5 123.6  GLU-317   ALA-318  22.7 20.5 -153.6 59.4 94.2 40.9 41.3  ALA-318   GLU-319  19.9 18.7 -12.9 -45.4 98.9 51.5 64.6  GLU-319   GLY-320  18.9 20.5 -26.0 150.4 174.4 60.5 172.3  GLY-320   GLY-321  19.9 20.9 -118.4 5.8 113.4 105.6 -131.3  GLY-321   VAL-322  17.7 18.3 -26.7 9.5 75.5 78.8 0.5  VAL-322   TYR-323  14.6 15.5 -14.1 7.1 40.0 43.7 19.9  VAL-322   TYR-323  14.6 15.5 -14.1 7.1 40.0 43.7 19.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-322   TYR-323  14.6 15.5 -14.1 7.1 40.0 43.7 19.9  VAL-322   TYR-323  14.6 15.5 -14.1 7.1 40.0 43.7 19.9  TYR-323   ASP-324  12.1 13.4 8.2 -11.8 73.7 75.2 -2.9  ASP-324   ILE-325  9.9 11.1 -7.3 -8.9 50.0 39.0 28.8  ILE-325   SER-326  6.4 7.9 3.5 -13.7 88.5 78.5 -5.8  SER-326   ASN-327  4.9 5.9 8.7 5.4 45.7 33.4 35.7  SER-326   ASN-327  4.9 5.9 8.7 5.4 45.7 33.4 35.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-326   ASN-327  4.9 5.9 8.7 5.4 45.7 33.4 35.7  SER-326   ASN-327  4.9 5.9 8.7 5.4 45.7 33.4 35.7  ASN-327   LYS-328  3.4 4.7 5.8 -4.9 33.4 58.8 21.8  LYS-328   ARG-329  4.2 7.1 -21.6 29.0 103.1 103.6 -1.0  ARG-329   ARG-330  5.7 7.9 14.8 -15.4 132.5 138.9 3.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees