Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PHE-165   TYR-166  16.9 16.6 3.9 -2.1 77.2 76.3 4.7
 TYR-166   ARG-167  17.4 17.1 7.9 -15.2 40.0 40.9 2.4
 ARG-167   ASP-168  14.8 14.4 11.7 6.7 36.8 37.2 -48.0
 ASP-168   GLN-169  15.0 13.0 -46.1 28.3 108.2 79.6 -42.7
 GLN-169   PRO-170  11.1 9.8 -43.8 -4.4 33.0 13.5 180.4
 PRO-170   HIS-171  8.6 8.2 1.9 25.0 129.6 122.3 -38.1
 HIS-171   PRO-172  7.8 7.8 -5.6 -2.0 107.9 104.5 -12.3
 PRO-172   PHE-173  5.8 6.0 0.7 -2.4 116.2 114.9 11.1
 PHE-173   ILE-174  3.1 3.0 -6.0 14.1 123.6 124.8 9.7
 ILE-174   THR-175  3.4 3.3 -4.2 12.7 149.2 150.3 18.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ARG-223   LYS-224  6.1 5.5 -1.1 12.3 147.9 152.9 23.8
 LYS-224   ALA-225  6.0 5.0 -11.4 8.0 110.5 112.5 -17.3
 ALA-225   SER-226  9.7 8.9 -23.4 10.0 107.8 110.8 -63.7
 GLN-229   SER-230  10.4 8.4 -13.4 -7.7 42.5 53.4 59.4
 SER-230   ASP-231  9.9 9.7 14.1 27.6 74.3 86.5 -63.4
 ASP-231   ASP-232  10.3 10.8 -83.7 67.0 30.3 27.9 62.0
 ASP-232   LEU-233  10.0 9.7 -6.5 -9.2 79.1 85.3 -1.6
 LEU-233   LEU-234  8.7 8.4 8.2 -8.3 75.0 74.6 -0.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees