Ubiquitin Carboxyl-Terminal Hydrolase 7

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  MET-328   VAL-329  3.3 2.1 10.4 -1.6 72.6 76.1 21.9  VAL-329   SER-330  4.4 2.8 -9.1 5.3 175.6 174.5 -18.8  SER-330   TYR-331  5.9 4.4 12.0 -13.1 68.5 77.8 4.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-341   ASP-342  13.1 12.1 27.1 -10.6 63.3 67.9 39.7  ASP-342   ARG-343  10.1 9.3 40.7 -48.7 39.7 66.7 -10.2  ARG-343   ARG-344  10.2 8.4 -13.4 -8.9 118.4 114.1 -37.0  ARG-344   GLU-345  7.8 5.9 6.3 -3.1 74.6 78.8 25.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-368   ALA-369  3.8 3.9 1.2 6.7 34.6 35.1 26.5  ALA-369   VAL-370  3.5 3.5 -8.0 1.6 98.2 97.6 15.8  VAL-370   GLU-371  0.5 1.8 10.3 -14.5 46.1 42.3 4.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-391   GLY-392  5.6 5.1 -12.2 2.0 104.3 100.9 -8.0  GLY-392   VAL-393  2.5 2.2 -2.4 9.6 164.9 163.8 22.1  VAL-393   LYS-394  2.1 0.4 4.3 -3.8 63.1 65.4 -5.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees