Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-86   PRO-87  2.2 2.5 17.2 -4.3 91.5 106.5 -21.1  PRO-87   GLU-88  4.4 4.7 -77.5 53.6 40.5 46.4 84.3  GLU-88   ILE-89  7.1 7.0 1.1 -11.9 100.8 92.3 -0.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-186   PRO-187  8.9 8.9 -11.2 7.2 57.1 63.6 0.9  PRO-187   ALA-188  7.6 7.4 -15.3 8.1 102.1 107.8 -7.6  ALA-188   VAL-189  5.5 4.9 14.4 -5.2 114.8 132.6 -10.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees