Bacteriophage T7 RNA Polymerase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-542   ILE-543  16.8 16.9 6.9 2.3 86.3 87.6 36.8  ILE-543   GLN-544  20.1 20.3 4.2 -4.8 101.8 104.4 37.9  GLN-544   HIS-545  18.2 18.3 -11.7 1.3 148.3 142.2 -72.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-708   GLU-709  22.0 21.6 27.5 -22.6 146.4 144.4 10.3  GLU-709   VAL-710  21.7 21.5 -1.0 34.7 41.0 49.2 -203.9  VAL-710   LYS-711  20.8 20.9 45.1 -14.1 136.8 105.6 -150.2  LYS-711   ASP-712  24.0 24.3 144.5 146.8 149.4 157.9 565.7  ASP-712   LYS-713  25.9 24.0 -25.4 -50.6 65.2 68.4 387.9  LYS-713   LYS-714  29.0 26.3 -158.7 -38.0 55.8 15.8 -1468.3  LYS-714   THR-715  29.0 24.7 -133.5 -62.3 67.8 59.9 797.0  THR-715   GLY-716  25.4 23.1 12.3 -126.5 16.5 119.7 899.4  GLY-716   GLU-717  25.5 19.4 83.4 112.5 82.2 58.5 -480.1  GLU-717   ILE-718  21.9 16.1 -141.7 -6.1 146.8 151.8 -1422.2  ILE-718   LEU-719  19.6 18.3 -27.2 -70.5 84.1 130.1 -175.7  LEU-719   ARG-720  16.6 18.4 -147.8 -31.6 146.5 139.2 1497.7  ARG-720   LYS-721  15.8 17.0 -9.5 8.3 125.2 128.0 -11.9  LYS-721   ARG-722  17.5 17.9 -50.7 16.0 155.5 164.0 -295.2  ARG-722   CYS-723  18.0 19.4 -29.1 -19.0 116.0 114.0 -291.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-724   VAL-725  13.6 14.3 4.2 -35.2 109.0 97.6 126.0  VAL-725   HIS-726  12.8 13.0 9.4 -14.1 116.0 131.2 88.9  HIS-726   TRP-727  9.1 9.7 14.8 3.5 111.7 106.9 -94.7  TRP-727   VAL-728  8.0 8.2 -11.3 9.6 54.2 50.1 -13.1  VAL-728   THR-729  5.5 5.6 -13.8 9.4 34.4 41.0 92.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-732   PHE-733  3.1 2.9 -4.5 2.3 95.3 97.0 14.4  PHE-733   PRO-734  1.5 1.4 10.9 -2.6 41.5 49.7 18.7  PRO-734   VAL-735  3.3 3.8 -7.6 16.6 79.2 73.8 6.0  VAL-735   TRP-736  7.0 7.6 -18.8 11.6 143.7 144.8 -66.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-770   ALA-771  23.2 24.1 -27.7 31.0 84.2 83.6 33.7  ALA-771   HIS-772  23.2 23.7 -2.2 7.1 39.0 38.2 -19.9  HIS-772   LYS-773  25.5 25.7 -7.7 6.1 36.7 44.7 14.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PHE-782   VAL-783  13.4 13.5 0.5 1.9 28.7 25.3 19.3  VAL-783   HIS-784  14.0 14.4 1.5 -5.7 59.5 53.8 26.3  HIS-784   SER-785  12.2 12.6 9.6 7.7 105.9 108.6 -61.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PHE-807   ALA-808  1.9 2.1 -4.4 0.3 83.3 80.9 -30.9  ALA-808   LEU-809  3.3 3.0 24.3 -13.3 45.3 49.5 40.5  LEU-809   ILE-810  4.6 5.0 -27.1 23.1 100.1 82.7 -43.9  ILE-810   HIS-811  8.2 8.6 45.8 -21.8 50.8 61.3 185.6  HIS-811   ASP-812  8.6 11.1 106.5 -154.5 97.4 79.1 89.9  ASP-812   SER-813  9.8 9.0 23.4 -15.3 169.1 139.1 -44.8  SER-813   PHE-814  8.6 8.2 3.4 -5.8 86.9 102.5 65.8  PHE-814   GLY-815  5.3 4.7 31.5 -16.6 158.1 140.5 -108.5  GLY-815   THR-816  3.3 3.4 5.5 29.4 98.3 101.2 -86.8  THR-816   ILE-817  1.6 2.2 16.2 -12.8 177.7 176.5 -15.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-821   ALA-822  7.3 7.4 -2.6 -4.2 110.9 112.7 -34.2  ALA-822   ASN-823  11.0 11.1 0.0 5.9 158.7 156.8 35.9  ASN-823   LEU-824  11.1 11.3 4.1 13.6 47.1 42.2 141.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PHE-849   ALA-850  12.4 13.2 -78.6 44.4 43.8 16.1 287.2  ALA-850   ASP-851  13.7 14.7 32.0 16.1 153.3 130.5 -349.3  ASP-851   GLN-852  11.1 11.2 16.7 -33.9 86.8 106.2 44.4  GLN-852   LEU-853  9.6 11.5 36.0 34.0 94.6 119.5 -148.5  LEU-853   HIS-854  13.2 14.7 19.2 -162.3 124.8 173.7 1299.9  HIS-854   GLU-855  16.4 15.6 -30.7 153.3 22.3 65.7 -1026.6  GLU-855   SER-856  16.3 15.1 -0.8 -51.1 58.7 112.4 -41.6  SER-856   GLN-857  19.5 18.5 82.6 -16.3 47.0 78.6 478.0  GLN-857   LEU-858  22.6 20.6 28.0 -138.5 48.1 14.9 -1127.0  LEU-858   ASP-859  21.1 20.1 -56.8 86.5 109.9 125.3 89.7  ASP-859   LYS-860  24.5 19.9 -110.7 166.5 122.5 103.7 421.6  LYS-860   MET-861  27.3 23.3 43.4 41.0 29.1 6.7 790.6  MET-861   PRO-862  24.7 21.8 -151.0 -37.4 125.0 123.9 -1506.4  PRO-862   ALA-863  23.2 24.1 -64.3 -151.2 128.7 105.8 419.3  ALA-863   LEU-864  25.2 25.1 59.1 -6.6 22.2 20.5 495.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees