Gdp-Mannose-Dependent Alpha-(1-2)-Phosphatidylinositol Mannosyltransferase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-114   ALA-115  11.1 10.9 4.3 -4.5 114.1 121.7 -1.2  ALA-115   THR-116  9.2 9.1 2.4 9.1 161.7 160.2 -1.8  THR-116   PHE-117  9.0 9.1 -8.2 18.5 70.4 72.3 -6.9  PHE-117   HIS-118  7.2 6.4 -30.9 27.4 12.0 10.9 5.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-143   ARG-144  7.4 10.0 150.2 -14.3 33.8 49.9 81.1  ARG-144   ILE-145  7.4 7.4 127.2 -51.9 36.0 23.8 48.4  ILE-145   ALA-146  4.4 8.6 173.0 -64.3 37.4 51.8 56.1  ALA-146   VAL-147  7.1 6.2 -165.7 9.7 120.7 150.6 -127.9  VAL-147   SER-148  9.2 7.2 5.6 -0.5 78.9 43.2 11.4  SER-148   ASP-149  7.9 5.8 15.8 7.7 72.1 118.3 -1.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-159   GLY-160  11.7 5.7 -147.9 -6.9 52.3 71.9 35.7  GLY-160   SER-161  11.3 9.1 15.2 -35.6 68.4 102.0 -2.3  SER-161   ASP-162  8.8 8.8 16.6 62.2 42.5 37.7 46.7  ASP-162   ALA-163  10.5 10.1 123.6 -80.2 33.3 72.6 1.4  ALA-163   VAL-164  7.9 8.4 35.5 5.1 30.3 43.8 23.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees