Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 TYR-166   ARG-167  16.3 16.1 5.5 -15.5 35.3 35.5 -15.8
 ARG-167   ASP-168  14.0 13.9 16.5 -0.1 38.1 33.5 -41.4
 ASP-168   GLN-169  14.5 13.0 -35.3 28.9 113.3 89.3 -22.1
 GLN-169   PRO-170  10.7 9.8 -36.6 -6.1 37.2 20.1 173.9
 PRO-170   HIS-171  7.9 7.7 3.6 11.8 127.4 122.0 -18.9
 HIS-171   PRO-172  6.9 7.2 -5.7 2.7 113.2 109.1 -16.2
 PRO-172   PHE-173  5.1 5.7 -1.6 -2.5 68.1 69.7 27.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-224   ALA-225  5.5 5.2 -4.3 15.8 113.6 122.9 7.8
 ALA-225   SER-226  8.6 8.4 -36.1 11.1 112.9 120.7 -92.9
 GLN-229   SER-230  6.9 5.2 2.5 0.9 136.0 128.2 -23.3
 SER-230   ASP-231  6.8 7.4 11.6 58.9 76.7 93.8 -54.2
 ASP-231   ASP-232  7.9 9.9 -80.0 51.0 30.3 40.0 124.0
 ASP-232   LEU-233  8.2 7.8 -17.2 -14.5 73.8 100.2 6.2
 LEU-233   LEU-234  7.9 7.5 17.6 -1.3 78.3 74.6 19.1
 LEU-234   THR-235  4.2 3.9 0.4 -10.5 17.2 5.6 -47.3
 THR-235   HIS-236  4.2 4.0 -2.7 18.8 103.8 110.3 34.1
 HIS-236   MET-237  7.6 7.4 -8.2 -16.2 63.1 84.3 3.4
 MET-237   LEU-238  7.3 7.1 9.8 17.0 56.2 56.9 8.3
 LEU-238   ASN-239  5.5 4.8 -5.2 25.3 163.3 158.4 106.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees