Hyb3 Light Chain

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-105   VAL-106  6.9 6.8 5.2 -0.8 100.0 97.1 -17.9  VAL-106   LEU-106A  10.2 10.1 8.2 13.3 130.8 125.4 -76.3  LEU-106A   GLY-107  11.0 11.1 18.3 -14.7 117.2 136.0 77.4  GLY-107   GLN-108  13.7 13.2 -10.9 12.6 51.8 36.1 33.9  GLN-108   PRO-109  12.4 11.7 -13.2 3.3 27.7 36.3 10.7  PRO-109   LYS-110  13.1 12.5 1.6 4.4 110.4 109.1 -0.6  LYS-110   ALA-111  11.2 11.0 0.2 -9.6 142.7 147.4 50.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-164   SER-165  4.9 5.1 10.1 -7.4 131.3 134.3 -31.5  SER-165   LYS-166  4.0 3.9 3.2 -8.7 76.2 72.5 12.3  LYS-166   GLN-167  3.2 3.0 -0.4 8.5 118.9 116.7 76.8  GLN-167   SER-168  5.9 5.7 -6.8 14.2 63.1 66.3 0.0  SER-168   ASN-170  9.2 9.2 -6.5 4.8 84.8 88.1 26.7  SER-168   ASN-170  9.2 9.2 -6.5 4.8 84.8 88.1 26.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-168   ASN-170  9.2 9.2 -6.5 4.8 84.8 88.1 26.7  SER-168   ASN-170  9.2 9.2 -6.5 4.8 84.8 88.1 26.7  ASN-170   ASN-171  9.1 9.0 -4.8 -6.1 116.0 120.3 -54.8  ASN-171   LYS-172  6.9 6.8 7.5 0.4 117.0 118.1 46.5  LYS-172   TYR-173  4.3 4.4 4.0 4.5 84.8 82.4 19.3  TYR-173   ALA-174  0.7 0.8 9.6 -16.9 147.7 146.3 42.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees