Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PHE-173   ILE-174  3.5 2.5 -1.7 8.5 118.9 120.3 13.7
 ILE-174   THR-175  0.7 0.4 -1.3 4.2 128.1 122.4 13.6
 THR-175   SER-176  3.5 2.8 -3.0 12.1 61.0 67.3 24.5
 SER-176   MET-177  6.1 5.5 -23.8 24.6 69.6 84.9 7.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-210   VAL-211  11.1 10.8 38.5 -34.3 101.8 103.8 -21.4
 VAL-211   MET-212  8.6 8.4 2.5 -10.1 57.9 59.0 1.4
 MET-212   ASN-213  5.7 5.2 40.8 -8.0 14.5 14.8 108.1
 ASN-213   ASP-214  7.3 6.9 16.7 -8.3 77.6 76.8 43.9
 ASP-214   LEU-215  8.8 8.3 -8.5 10.2 92.0 96.0 13.9

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees