RNA Helicase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-332   HIS-333  7.1 5.2 43.0 -49.5 70.6 39.7 -10.0  HIS-333   PRO-334  6.5 5.2 -5.6 -10.6 138.6 102.3 -43.5  PRO-334   ASN-335  7.8 8.5 64.4 -12.3 91.3 76.0 85.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-433   GLN-434  4.0 4.1 -22.9 46.6 165.2 160.9 135.4  GLN-434   THR-435  2.3 2.9 -9.4 7.6 90.8 99.2 -9.7  THR-435   VAL-436  1.3 0.9 -1.5 5.6 164.5 160.0 22.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PHE-444   THR-445  4.1 3.2 -1.2 0.2 100.3 97.9 -2.1  THR-445   ILE-446  0.8 0.5 -16.2 25.6 168.0 164.5 63.2  ILE-446   GLU-447  1.4 2.5 11.7 -43.4 80.8 81.2 -62.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-480   ARG-481  8.8 9.1 71.8 -37.2 55.6 69.2 87.1  ARG-481   PRO-482  7.7 7.9 -6.4 -9.3 62.3 56.1 4.6  PRO-482   SER-483  7.0 6.7 15.5 17.9 75.3 58.3 54.8  SER-483   GLY-484  4.4 4.3 -1.6 4.4 108.4 112.9 25.9  GLY-484   MET-485  6.3 6.7 43.2 -36.8 20.0 23.9 49.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees