Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 MET-5   PRO-6  6.9 6.6 18.1 3.2 76.4 68.4 24.7
 PRO-6   GLN-7  6.5 6.4 -1.0 7.9 134.8 133.1 20.9
 GLN-7   PRO-8  5.7 5.5 -2.2 -9.4 101.8 97.4 -9.1
 PRO-8   LYS-9  7.8 7.9 10.6 -1.0 27.8 21.8 64.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-17   PRO-18  8.8 8.4 -6.4 -6.3 95.7 97.7 -17.2
 PRO-18   LEU-19  12.0 11.8 10.7 21.3 19.3 19.0 156.5
 LEU-19   LEU-20  11.9 11.6 -22.7 33.4 111.4 109.5 25.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLU-38   ILE-39  1.4 1.5 -6.3 8.5 145.5 145.6 1.6
 ILE-39   PHE-40  1.1 1.0 3.4 6.4 39.5 40.1 17.9
 PHE-40   LYS-41  1.8 1.8 7.3 -6.0 38.7 39.6 -5.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLY-46   ARG-47  5.3 5.5 51.5 -11.0 26.5 25.9 196.6
 ARG-47   VAL-48  5.3 6.0 -12.8 -0.9 137.5 133.8 -109.1
 VAL-48   THR-49  3.9 4.4 5.5 12.1 34.5 33.0 53.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees