Igg2a Intact Antibody - Mab231

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ILE-232   LYS-235  18.3 17.6 -37.3 -22.0 104.2 90.4 -24.8
 LYS-235   PRO-236  14.4 16.6 166.9 -3.2 145.3 90.3 -84.4
 PRO-236   CYS-237  12.1 13.0 79.8 136.7 101.8 119.8 44.5
 CYS-237   PRO-238  9.0 9.7 52.0 25.5 97.4 44.7 23.1
 PRO-238   PRO-239  8.8 8.7 -105.5 33.8 64.8 102.5 10.9
 PRO-239   CYS-240  6.1 5.5 -25.5 70.7 90.8 31.6 -21.0
 CYS-240   LYS-241  2.3 2.8 55.5 125.5 128.6 91.7 -70.4
 LYS-241   CYS-242  2.8 4.6 5.3 -118.4 138.6 46.2 12.0
 CYS-242   PRO-243  0.9 4.3 28.9 -6.9 147.1 76.1 -28.6
 PRO-243   ALA-244  3.6 7.9 121.0 7.5 129.0 15.5 61.0
 ALA-244   PRO-245  2.2 10.0 -12.6 25.5 31.0 122.6 -3.0
 PRO-245   ASN-246  3.5 8.4 62.8 -6.0 119.0 96.4 86.6
 ASN-246   LEU-247  2.1 9.2 -152.3 7.0 70.8 137.8 -145.8
 LEU-247   LEU-248  5.6 8.7 -18.0 -59.5 77.2 146.1 143.8
 LEU-248   GLY-249  8.4 8.1 44.4 -54.4 175.5 43.9 -8.6
 GLY-249   GLY-250  8.9 8.5 144.8 70.0 115.4 60.1 42.1
 GLY-250   PRO-251  12.2 12.0 45.3 7.9 94.2 93.2 4.2

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Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees