Aspartate Aminotransferase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASN-34   LEU-35  12.9 12.9 -10.9 17.9 102.5 100.1 28.2  LEU-35   GLY-36  9.9 9.8 -14.5 -6.1 144.9 136.1 -155.2  GLY-36   VAL-37  10.5 10.6 92.0 -6.0 92.5 108.3 -77.6  VAL-37   GLY-38  10.2 10.0 6.2 -42.9 122.0 65.3 64.9  GLY-38   ALA-39  7.4 8.9 -63.9 -6.8 39.7 52.3 450.0  ALA-39   TYR-40  8.7 8.6 27.9 6.6 135.6 133.9 -203.1  TYR-40   ARG-41  6.6 6.4 -1.6 -2.7 45.5 46.5 0.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-328   ARG-329  6.1 6.4 -2.0 3.3 92.2 91.1 26.1  ARG-329   ILE-330  2.7 3.0 -1.0 -2.5 29.4 25.3 38.7  ILE-330   LEU-331  2.6 3.0 -1.6 -3.1 79.9 76.7 -3.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLN-356   ILE-357  6.9 7.4 6.5 -0.3 142.1 142.8 -68.7  ILE-357   GLY-358  6.0 6.5 -25.1 0.4 95.3 87.7 73.6  GLY-358   MET-359  4.2 4.2 -7.6 12.0 60.9 73.9 82.3  MET-359   PHE-360  1.7 1.3 -17.3 14.2 117.2 129.0 -44.9  PHE-360   SER-361  1.3 1.0 -25.1 -7.9 104.6 111.1 -118.2  SER-361   PHE-362  4.4 3.6 0.8 9.4 34.2 42.4 47.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees