Nicotinamide Phosphoribosyltransferase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-241   VAL-242  11.2 11.6 -10.5 -0.5 101.0 105.0 -2.6  VAL-242   PRO-243  7.6 8.1 9.1 0.5 32.9 36.4 57.8  PRO-243   ALA-244  5.8 6.1 -6.4 -6.1 76.4 77.9 17.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-310   ARG-311  7.7 7.4 11.9 -36.4 87.0 85.9 -44.0  ARG-311   PRO-312  10.3 9.9 10.6 -41.1 93.2 112.6 -49.8  PRO-312   ASP-313  13.6 13.6 -7.9 -75.9 43.4 72.0 462.7  ASP-313   SER-314  16.7 15.8 -159.1 -69.8 97.4 77.3 72.2  SER-314   GLY-315  19.6 19.3 25.2 -68.7 52.9 39.5 -225.6  GLY-315   ASN-316  22.4 21.8 -49.3 23.3 138.7 145.4 -158.3  ASN-316   PRO-317  22.7 22.9 -15.9 -1.8 106.8 110.5 -52.2  PRO-317   LEU-318  19.8 19.8 17.0 -13.1 38.9 29.2 18.0  LEU-318   ASP-319  19.4 20.0 -0.9 -5.2 80.4 85.3 34.6  ASP-319   THR-320  20.7 21.4 13.8 -11.5 125.4 123.3 2.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-329   LYS-330  13.5 13.4 -10.7 -2.3 89.5 92.5 -23.4  LYS-330   LYS-331  16.9 16.8 43.4 -32.8 59.7 42.7 33.3  LYS-331   PHE-332  15.7 15.4 1.9 -11.4 106.9 103.4 6.5  PHE-332   PRO-333  12.8 12.6 -16.3 22.2 2.9 5.4 -46.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees