Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ALA-110   LEU-111  19.6 19.6 16.9 -23.4 103.3 110.2 1.5
 LEU-111   ASP-112  16.5 16.4 -0.3 -5.1 116.9 113.8 6.3
 ASP-112   THR-113  13.4 13.4 3.5 -18.8 121.2 117.4 -16.0
 THR-113   LYS-114  10.2 10.3 -0.1 14.4 111.0 121.9 7.9
 LYS-114   GLY-115  7.6 8.1 14.9 10.8 74.4 79.6 11.4
 GLY-115   PRO-116  4.6 4.9 -14.5 11.6 92.6 77.0 0.1
 PRO-116   GLU-117  0.7 1.2 76.1 -22.6 35.2 39.2 104.0
 GLU-117   ILE-118  2.9 3.3 -13.4 -2.8 79.1 81.8 -5.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ALA-214   VAL-215  10.4 11.2 28.0 -0.8 38.0 40.8 58.0
 VAL-215   ASP-216  7.8 8.4 -13.9 52.9 132.3 155.5 113.0
 ASP-216   LEU-217  7.2 8.8 -110.7 65.0 120.6 115.9 -90.8
 LEU-217   PRO-218  3.7 4.9 16.3 -13.1 64.4 61.1 10.2
 PRO-218   ALA-219  3.1 4.4 28.8 -11.8 121.8 124.7 -16.7
 ALA-219   VAL-220  4.8 6.2 -9.6 5.5 114.2 88.0 -15.0
 VAL-220   SER-221  4.4 4.9 48.6 -35.1 60.5 72.5 -4.0
 SER-221   GLU-222  3.0 3.0 15.0 -2.8 27.3 29.7 43.4
 GLU-222   LYS-223  4.6 4.6 6.5 -7.0 111.7 102.5 6.6
 LYS-223   ASP-224  7.5 7.9 2.4 -5.1 106.1 101.2 9.4
 ASP-224   ILE-225  7.4 7.9 22.0 -9.4 45.1 35.4 26.3
 ILE-225   GLN-226  8.6 8.3 -8.9 -10.9 101.9 109.2 3.1
 GLN-226   ASP-227  10.6 11.0 24.2 -5.8 129.8 131.7 -33.6
 ASP-227   LEU-228  12.2 12.5 -2.5 -8.0 97.8 94.8 -13.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees