5-Aminolevulinate Synthase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-314   LYS-315  3.9 3.7 3.8 -5.9 64.6 65.5 20.9  LYS-315   MET-316  3.7 3.5 1.5 4.9 71.8 75.7 -8.8  MET-316   ARG-317  6.9 6.8 -0.5 1.2 42.0 37.2 23.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-324   ILE-325  11.9 12.0 -36.3 27.9 88.8 78.1 -8.1  ILE-325   ILE-326  8.6 8.7 5.5 -18.1 136.5 145.1 151.1  ILE-326   ASP-327  9.2 9.3 16.1 -8.2 113.3 113.3 -45.1  ASP-327   HIS-328  6.2 6.3 0.1 3.1 146.3 149.7 -52.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-332   VAL-333  6.1 6.4 -7.4 5.2 92.2 90.6 -27.5  VAL-333   PRO-334  4.2 4.5 -4.1 0.3 55.1 52.9 27.9  PRO-334   VAL-335  7.5 7.8 12.1 -13.6 92.4 96.4 111.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-373   ARG-374  8.7 8.9 0.8 -13.6 138.5 142.1 98.2  ARG-374   PHE-375  7.6 7.7 -9.4 17.5 108.0 101.2 30.2  PHE-375   THR-376  4.4 4.3 -2.8 0.7 51.4 49.7 -5.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees