Sugar Abc Transporter, Periplasmic Sugar-Binding Protein

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-101   GLY-102  6.1 6.5 -16.9 15.3 42.1 44.0 11.2  GLY-102   THR-103  3.6 3.8 21.7 -23.6 103.8 105.6 28.7  THR-103   ASP-104  0.5 0.3 23.4 3.1 45.3 45.4 51.6  ASP-104   ASN-105  3.1 3.0 -9.6 0.3 58.0 69.4 17.6  ASN-105   TYR-106  5.4 5.1 -8.3 -0.6 95.2 93.3 -1.7  TYR-106   GLN-107  8.4 8.2 -1.6 10.6 54.5 53.9 -13.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  MET-234   GLY-235  4.9 5.0 -12.8 17.0 89.6 88.9 7.2  GLY-235   GLN-236  1.5 1.3 -1.8 -6.1 27.7 26.8 31.7  GLN-236   ARG-237  1.2 1.1 18.1 -5.8 80.6 72.0 24.4  ARG-237   PRO-238  4.9 4.8 -1.8 -2.6 60.3 69.7 19.4  PRO-238   TYR-239  7.0 7.1 -0.1 0.7 86.4 86.8 -11.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-278   THR-279  5.2 5.1 3.8 -12.2 148.8 151.5 25.0  THR-279   GLY-280  5.8 5.5 0.4 28.2 134.5 137.9 -53.8  GLY-280   VAL-281  5.5 5.1 -32.2 -13.8 28.2 51.5 103.2  VAL-281   ASP-282  4.8 3.9 -5.9 -31.5 39.4 46.0 33.3  ASP-282   VAL-283  6.0 5.4 -6.2 19.4 63.8 63.1 -18.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees