S-Arrestin

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-174   GLU-175  3.9 3.9 -2.3 -9.3 30.6 34.8 68.4  GLU-175   LYS-176  3.2 3.2 -12.4 27.4 58.9 55.2 -76.0  LYS-176   VAL-177  4.4 5.2 4.0 -14.2 157.6 150.1 54.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-293   ALA-294  13.0 13.5 -19.9 6.0 56.0 59.8 71.0  ALA-294   LEU-295  9.7 10.0 11.1 -16.9 88.9 88.8 10.4  LEU-295   ASP-296  7.5 7.7 -32.8 47.3 66.8 65.0 -71.8  ASP-296   GLY-297  4.2 4.2 122.2 108.5 155.6 162.4 688.1  GLY-297   LYS-298  5.8 3.8 -42.8 -38.7 67.7 70.5 -56.5  LYS-298   ILE-299  8.8 5.7 21.9 -9.2 51.3 140.9 -111.8  ILE-299   LYS-300  11.8 5.7 -58.6 -38.9 82.3 42.9 -839.4  LYS-300   HIS-301  13.9 8.4 93.8 137.9 89.3 64.5 -227.6  HIS-301   GLU-302  12.3 11.2 179.9 40.8 26.9 39.2 992.6  GLU-302   ASP-303  13.8 11.9 -160.4 16.7 135.4 148.4 -795.7  ASP-303   THR-304  11.3 12.6 117.3 -49.5 78.1 59.5 133.9  THR-304   ASN-305  9.1 9.5 53.1 -3.7 9.9 16.3 258.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  MET-321   GLY-322  12.6 15.2 -177.5 31.4 63.3 88.6 474.1  GLY-322   ILE-323  13.1 12.4 -99.1 29.5 127.6 140.7 -270.3  ILE-323   LEU-324  11.7 10.9 -5.1 -17.3 151.7 138.1 -86.9  LEU-324   VAL-325  11.5 10.3 5.3 2.1 62.4 43.8 54.2  VAL-325   SER-326  9.0 8.5 -3.9 10.9 159.5 154.0 -0.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees