Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PHE-173   ILE-174  12.2 12.7 3.6 2.7 83.0 77.4 20.1
 ILE-174   THR-175  14.2 14.8 -0.3 3.6 69.2 63.3 -32.1
 THR-175   SER-176  17.6 18.1 -11.1 1.3 5.1 14.6 103.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-218   ILE-219  6.1 6.3 1.5 -5.1 122.5 118.8 104.4
 ILE-219   ILE-220  3.0 3.0 -4.9 7.7 119.3 123.0 -16.5
 ILE-220   ALA-221  3.5 3.3 2.7 0.3 86.2 88.8 -78.5
 ALA-221   ASP-222  7.0 7.0 3.2 -3.9 148.2 147.9 7.3
 ASP-222   ARG-223  7.9 7.5 -3.7 7.6 78.5 85.9 78.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 SER-230   ASP-231  11.1 10.8 -7.0 12.3 101.0 113.6 -121.8
 ASP-231   ASP-232  11.5 11.9 -56.7 58.9 75.1 83.3 111.9
 ASP-232   LEU-233  9.1 9.1 2.3 -5.3 34.1 35.1 32.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ILE-258   ILE-259  5.8 5.9 -3.0 1.6 104.8 104.5 17.0
 ILE-259   THR-260  8.2 8.4 5.6 -5.3 63.4 64.9 17.6
 THR-260   PHE-261  11.0 11.1 -8.4 11.7 164.8 167.2 67.9

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ILE-263   ALA-264  12.0 12.4 -8.6 2.0 62.6 56.6 84.1
 ALA-264   GLY-265  13.8 14.0 -0.8 1.5 27.9 28.1 -54.3
 GLY-265   HIS-266  11.5 11.5 -3.4 -5.5 38.9 40.6 126.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees