Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-86   PRO-87  2.9 2.5 27.6 -20.9 84.6 93.3 -7.0  PRO-87   GLU-88  3.0 3.3 -72.5 32.2 42.1 43.9 135.6  GLU-88   ILE-89  6.2 6.1 12.5 -9.2 90.5 77.3 -48.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-177   ASN-178  10.9 11.3 -30.9 15.2 106.1 105.5 1.4  ASN-178   LEU-179  8.3 8.7 -26.3 17.1 113.1 108.6 -12.5  LEU-179   PRO-180  9.2 9.7 -4.7 16.8 131.9 124.3 -27.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-181   CYS-182  10.2 9.2 26.6 -16.0 32.6 46.7 -18.0  CYS-182   ASP-183  10.7 10.9 -3.9 1.7 126.0 138.6 -14.2  ASP-183   VAL-184  10.4 10.8 -17.7 8.6 152.6 150.4 -44.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-186   PRO-187  7.2 7.2 -0.4 0.8 66.3 73.3 -12.9  PRO-187   ALA-188  6.1 5.3 -31.1 16.8 110.9 113.2 -34.8  ALA-188   VAL-189  4.8 4.0 -14.1 9.0 64.2 52.8 28.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees