Pyruvate Kinase Isozymes M1/m2

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 THR-113   LYS-114  9.5 9.6 -3.2 7.9 56.6 56.9 -10.9
 LYS-114   GLY-115  8.0 8.1 -5.4 -1.8 78.2 75.6 1.4
 GLY-115   PRO-116  4.3 4.4 12.6 0.3 86.0 93.4 10.9
 PRO-116   GLU-117  2.5 2.5 -65.1 49.0 31.0 38.4 56.1
 GLU-117   ILE-118  5.4 5.6 -19.6 11.9 99.7 100.4 46.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASP-216   LEU-217  8.5 7.9 -0.7 -8.5 51.2 47.2 37.4
 LEU-217   PRO-218  5.3 4.8 -3.1 1.9 61.1 67.6 -9.0
 PRO-218   ALA-219  3.6 2.7 3.4 -8.2 60.0 58.4 -4.7
 ALA-219   VAL-220  4.1 3.1 -0.9 2.6 78.4 65.3 2.1
 VAL-220   SER-221  3.0 3.1 -20.2 -5.9 60.7 56.4 55.0
 SER-221   GLU-222  2.4 2.4 -0.6 -8.3 29.6 37.8 26.4
 GLU-222   LYS-223  4.3 4.4 2.6 8.9 83.3 76.4 -22.1
 LYS-223   ASP-224  7.5 7.6 -8.0 0.5 100.4 105.1 -2.1
 ASP-224   ILE-225  6.8 6.8 5.1 -5.3 144.6 141.1 10.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees