Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PRO-170   HIS-171  3.4 3.1 2.0 -1.8 148.8 149.6 -1.5
 HIS-171   PRO-172  4.2 3.5 -6.4 6.9 81.8 79.8 -22.9
 PRO-172   PHE-173  4.5 3.6 -4.5 -1.6 58.3 63.4 23.1
 PHE-173   ILE-174  1.8 1.1 -10.4 16.6 126.9 133.5 20.9
 ILE-174   THR-175  1.4 2.0 -4.9 10.2 121.6 120.4 2.8
 THR-175   SER-176  3.7 3.3 -10.5 -6.0 56.5 61.7 18.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASN-213   ASP-214  5.2 5.3 5.5 -7.8 73.1 74.5 1.5
 ASP-214   LEU-215  5.6 5.4 8.3 -5.1 95.8 96.5 12.7
 LEU-215   VAL-216  2.8 2.3 4.6 4.9 41.7 35.8 31.4
 VAL-216   ASP-217  0.3 0.8 1.9 -4.2 32.5 41.2 -2.1
 ASP-217   LYS-218  2.8 3.1 -1.6 11.8 91.3 88.9 11.0
 LYS-218   ILE-219  5.0 4.8 -3.6 -1.8 96.8 107.7 9.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees