O-Succinylbenzoate Synthase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-130   LYS-131  9.0 9.0 -7.1 2.2 144.9 149.4 -60.9  LYS-131   VAL-132  9.9 9.8 -1.6 3.1 99.2 93.5 105.1  VAL-132   LYS-133  13.1 13.3 1.9 11.1 41.6 35.5 128.6  LYS-133   VAL-134  14.7 15.0 3.0 -1.3 48.8 51.9 -125.3  VAL-134   GLY-135  16.2 16.3 2.1 -11.3 54.4 53.7 -37.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PHE-223   VAL-224  10.7 10.4 15.0 -5.5 133.2 133.3 -86.1  VAL-224   ALA-225  9.1 8.2 -16.8 -13.9 100.3 107.1 -66.6  ALA-225   GLU-226  7.3 6.8 -8.8 -6.7 47.8 34.7 192.8  GLU-226   GLU-227  3.8 4.0 17.5 3.1 72.8 71.5 36.2  GLU-227   GLY-228  3.6 4.1 5.5 -1.4 64.1 65.8 -31.6  GLY-228   VAL-229  3.3 3.4 -6.8 1.0 138.0 134.0 -108.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ARG-246   GLU-247  15.5 15.7 1.9 -2.8 25.5 24.6 -25.8  GLU-247   GLN-248  16.6 16.7 0.5 -0.9 61.2 61.5 -1.8  GLN-248   VAL-249  13.3 13.4 0.1 1.0 112.0 109.5 22.7  VAL-249   GLN-250  11.2 11.4 4.1 1.0 69.1 65.4 139.4  GLN-250   ALA-251  13.5 13.9 2.3 -4.6 16.3 16.9 -126.7  ALA-251   ALA-252  13.0 13.0 -4.4 1.6 96.3 95.7 -40.8  ALA-252   HIS-253  9.3 9.3 4.2 -10.4 105.7 101.2 21.4  HIS-253   ALA-254  10.4 10.6 -0.2 5.7 130.4 134.7 8.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees