Vinculin Isoform Vcl

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-21   HIS-22  16.0 16.9 -12.7 -8.1 165.1 136.1 -58.0  HIS-22   LEU-23  16.8 19.5 -3.5 -4.1 125.6 150.5 -23.6  LEU-23   VAL-24  13.2 16.9 66.0 -78.9 83.1 75.5 21.3  VAL-24   ILE-25  13.8 16.4 -17.9 -0.4 131.8 101.0 -24.9  ILE-25   MET-26  17.0 20.0 17.9 -8.2 16.0 43.9 25.6  MET-26   HIS-27  15.3 21.5 29.3 -26.5 75.8 25.1 -0.4  HIS-27   GLU-28  12.6 19.3 70.4 -43.2 72.9 69.0 47.0  GLU-28   GLU-29  15.7 22.1 -0.1 1.6 147.2 118.7 18.1  GLU-29   GLY-30  17.2 24.5 12.0 -172.7 40.0 23.5 -393.3  GLY-30   GLU-31  13.9 22.4 69.6 12.8 84.5 87.4 -13.7  GLU-31   VAL-32  14.3 20.6 178.3 -0.8 71.2 45.2 529.3  VAL-32   ASP-33  18.1 18.9 -139.4 -0.8 145.0 77.3 -447.2  ASP-33   GLY-34  19.1 15.5 -171.9 102.6 141.7 105.0 455.8  GLY-34   LYS-35  15.9 15.5 172.2 -104.2 71.2 117.0 -178.0  LYS-35   ALA-36  16.0 14.4 25.6 -94.3 94.8 95.0 -63.2  ALA-36   ILE-37  13.3 15.5 -47.4 10.7 29.9 73.7 128.5  ILE-37   PRO-38  12.8 13.2 -164.8 5.7 102.3 68.1 56.8  PRO-38   ASP-39  14.4 16.2 135.7 -10.2 120.4 167.3 -347.2  ASP-39   LEU-40  13.6 17.0 -94.2 -21.6 7.0 58.1 325.0  LEU-40   THR-41  12.7 13.4 -19.4 -27.7 62.0 84.6 27.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-130   VAL-131  7.1 6.2 -7.2 5.6 145.5 136.9 -16.0  VAL-131   ARG-132  5.6 4.8 4.5 1.3 51.8 48.6 13.3  ARG-132   LYS-133  3.0 2.1 -9.2 3.9 122.3 130.8 -9.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees