Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-86   PRO-87  2.9 2.5 30.8 -23.5 84.6 93.7 -6.8  PRO-87   GLU-88  2.9 3.3 -72.6 31.7 42.0 44.8 138.4  GLU-88   ILE-89  6.2 6.1 11.0 -7.1 90.5 77.9 -49.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-177   ASN-178  10.8 11.3 -30.7 15.9 106.0 105.1 3.5  ASN-178   LEU-179  8.3 8.7 -25.3 17.2 113.1 108.0 -13.8  LEU-179   PRO-180  9.1 9.6 -5.3 17.9 131.8 124.9 -27.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-181   CYS-182  10.2 9.2 26.8 -17.2 32.6 46.3 -26.5  CYS-182   ASP-183  10.7 10.8 -5.4 4.0 126.0 138.9 -6.4  ASP-183   VAL-184  10.3 10.7 -17.7 8.8 152.5 149.8 -48.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-186   PRO-187  7.1 7.1 -0.3 0.6 66.3 72.4 -11.0  PRO-187   ALA-188  6.0 5.2 -31.6 17.2 110.9 113.5 -36.0  ALA-188   VAL-189  4.8 3.9 -13.2 7.8 64.3 52.1 31.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees