Pyruvate Kinase Isozymes M1/m2

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 THR-113   LYS-114  7.3 7.3 -6.3 1.9 46.9 50.5 -0.7
 LYS-114   GLY-115  7.3 7.4 -5.7 -13.4 72.6 73.1 13.1
 GLY-115   PRO-116  3.8 3.9 16.1 -1.5 89.3 107.6 -16.5
 PRO-116   GLU-117  5.3 5.1 -81.9 53.8 25.1 31.0 105.8
 GLU-117   ILE-118  8.0 7.3 5.5 -11.3 92.4 88.4 0.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-217   PRO-218  8.3 8.5 -5.8 2.1 49.4 61.4 -2.6
 PRO-218   ALA-219  6.5 6.3 -9.2 -6.1 111.4 114.1 -7.4
 ALA-219   VAL-220  3.8 3.4 10.5 -9.7 102.8 123.7 -3.2
 VAL-220   SER-221  1.4 1.6 -27.1 -2.5 70.4 58.8 72.6
 SER-221   GLU-222  4.8 4.8 7.3 -11.1 154.9 150.5 3.4
 GLU-222   LYS-223  6.5 5.9 0.2 8.8 90.9 81.2 -10.9

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees