Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-115   PRO-116  10.1 9.7 4.7 -0.2 103.8 102.9 -17.4  PRO-116   GLU-117  7.0 6.2 -5.3 45.0 125.8 124.4 79.0  GLU-117   ILE-118  4.7 4.6 -18.1 1.5 60.0 63.1 5.1  ILE-118   ARG-119  3.1 2.4 14.1 -18.6 72.5 69.6 9.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-208   ASN-209  3.9 3.7 6.6 -0.5 102.9 104.1 5.3  ASN-209   LEU-210  1.2 1.5 7.2 1.9 93.7 88.3 2.2  LEU-210   PRO-211  4.2 4.9 1.2 -1.8 119.4 120.1 -14.2  PRO-211   GLY-212  5.1 6.1 -141.8 172.1 96.0 99.0 63.6  GLY-212   ALA-213  8.6 9.8 11.4 -39.2 14.8 28.6 -62.7  ALA-213   ALA-214  8.2 9.5 -19.6 29.0 122.5 107.0 -36.7  ALA-214   VAL-215  9.0 10.6 22.4 7.4 38.1 40.3 87.1  VAL-215   ASP-216  6.1 7.4 -20.0 13.4 113.6 133.7 31.7  VAL-215   ASP-216  6.1 7.4 -20.0 13.4 113.6 133.7 31.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-215   ASP-216  6.1 7.4 -20.0 13.4 113.6 133.7 31.7  VAL-215   ASP-216  6.1 7.4 -20.0 13.4 113.6 133.7 31.7  ASP-216   LEU-217  7.5 8.5 -74.8 52.2 115.6 122.9 -25.1  LEU-217   PRO-218  6.6 6.3 16.1 -12.1 83.6 90.1 8.3  PRO-218   ALA-219  9.5 9.3 -3.9 7.4 44.2 40.4 -0.4  ALA-219   VAL-220  12.0 11.5 -10.3 1.0 112.2 109.6 -8.7  VAL-220   SER-221  12.6 12.5 14.6 -40.5 42.8 31.9 -105.6  SER-221   GLU-222  10.0 10.0 12.5 12.6 38.6 45.9 82.4  GLU-222   LYS-223  11.1 10.9 2.4 -10.0 114.6 111.7 30.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees