5' Polynucleotide Kinase-3' Phosphatase Catalytic Domain

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PHE-335   GLU-336  9.0 9.0 3.0 2.9 156.4 152.0 -58.3  GLU-336   LEU-337  9.7 9.9 1.5 -10.8 127.5 128.8 83.0  LEU-337   PRO-338  7.7 7.8 3.6 8.4 127.4 135.1 -55.3  PRO-338   ALA-339  8.9 8.7 -2.7 -12.3 47.9 57.4 75.8  ALA-339   PHE-340  11.5 11.3 14.9 -15.9 146.6 146.4 23.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees