Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PHE-165   TYR-166  16.8 16.5 10.0 -7.5 80.3 83.6 -5.9
 TYR-166   ARG-167  17.2 17.0 3.5 -12.9 31.5 35.3 -2.0
 ARG-167   ASP-168  15.1 14.9 12.6 1.1 39.8 31.6 -21.4
 ASP-168   GLN-169  15.9 14.3 -37.7 22.7 117.4 94.7 -61.0
 GLN-169   PRO-170  12.1 10.9 -38.0 -6.9 40.7 19.4 187.0
 PRO-170   HIS-171  9.3 8.9 14.0 4.8 125.1 118.3 -35.4
 HIS-171   PRO-172  7.8 7.9 -1.3 5.2 117.6 112.8 -12.8
 PRO-172   PHE-173  5.7 6.2 -4.0 -5.4 71.4 71.1 24.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-224   ALA-225  5.3 5.0 15.2 -1.1 64.3 65.2 33.8
 ALA-225   SER-226  8.2 8.2 7.6 -30.5 62.8 61.5 -89.2
 GLN-229   SER-230  5.9 4.8 0.3 5.0 134.1 123.0 -29.6
 SER-230   ASP-231  6.2 7.2 12.8 51.3 78.6 93.0 -50.4
 ASP-231   ASP-232  7.7 9.9 -75.8 45.6 31.2 34.0 136.9
 ASP-232   LEU-233  8.3 8.1 -14.3 -17.8 69.5 98.0 9.0
 LEU-233   LEU-234  8.2 7.8 17.4 -1.0 81.3 75.9 14.4
 LEU-234   THR-235  4.6 4.3 -0.4 -4.8 158.6 174.8 -24.8
 THR-235   HIS-236  4.6 4.4 -6.1 19.5 100.4 108.0 -0.1
 HIS-236   MET-237  8.0 7.8 -16.9 -9.7 58.8 80.2 20.4
 MET-237   LEU-238  7.8 7.8 11.8 17.5 59.0 56.6 9.8
 LEU-238   ASN-239  6.1 5.5 -7.0 23.4 159.5 156.3 80.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees