Nad-Dependent Malic Enzyme, Mitochondrial

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-88   GLN-89  14.6 14.7 -20.1 -2.8 74.1 69.7 248.5  GLN-89   GLU-90  11.2 11.4 55.6 -32.0 64.6 57.3 207.0  GLU-90   ARG-91  12.1 12.3 -16.7 -5.9 87.3 86.4 46.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-99   LEU-100  14.5 14.9 -6.9 4.7 92.0 92.6 16.5  LEU-100   GLN-101  11.5 11.9 29.1 -17.4 24.4 25.4 220.2  GLN-101   ASP-102  10.8 11.5 -21.1 8.7 115.2 116.0 -122.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-119   CYS-120  9.9 9.9 -11.9 10.5 115.3 124.1 -4.3  CYS-120   SER-121  7.8 7.8 9.0 -2.2 19.0 21.9 123.1  SER-121   GLN-122  10.1 10.2 -12.7 20.7 125.8 123.4 36.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ARG-194   PRO-195  7.6 7.1 13.6 -19.2 172.5 172.6 124.6  PRO-195   ASP-196  7.2 6.9 23.5 -7.3 118.9 121.0 -245.5  ASP-196   ARG-197  10.4 10.1 -19.1 8.9 104.4 92.8 10.5  ARG-197   CYS-198  9.7 9.4 30.9 -9.4 119.4 115.8 -180.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-478   ILE-479  10.9 10.8 -7.1 0.0 77.2 77.3 19.6  ILE-479   LEU-480  10.1 9.9 30.9 -13.8 38.8 36.3 223.9  LEU-480   CYS-481  10.4 9.8 -13.7 3.7 124.2 121.7 -146.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-483   ARG-484  15.8 15.7 6.6 -19.4 27.9 23.0 -185.6  ARG-484   HIS-485  18.5 18.1 13.3 -3.5 56.7 62.5 93.3  HIS-485   ILE-486  18.5 18.5 5.2 -27.3 114.2 117.7 179.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-554   GLU-555  3.8 3.7 -10.1 7.1 82.4 81.2 92.7  GLU-555   ARG-556  7.4 7.4 24.8 -14.5 163.8 157.3 -154.2  ARG-556   THR-557  7.5 7.6 -46.5 45.7 43.3 40.8 97.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees