Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 ALA-110   LEU-111  19.6 19.7 18.1 -26.4 102.7 107.3 1.6
 LEU-111   ASP-112  16.5 16.5 3.2 -3.0 61.7 66.2 0.6
 ASP-112   THR-113  13.4 13.4 -1.3 -15.3 57.6 60.3 -6.0
 THR-113   LYS-114  10.3 10.4 -6.4 21.3 109.3 121.8 2.7
 LYS-114   GLY-115  7.6 8.1 12.9 6.7 75.1 79.7 3.9
 GLY-115   PRO-116  4.7 4.9 -5.7 1.0 93.4 79.0 4.7
 PRO-116   GLU-117  0.8 1.3 71.2 -15.3 33.5 39.9 111.2
 GLU-117   ILE-118  2.9 3.2 -13.0 -6.9 78.7 81.1 -10.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 ALA-214   VAL-215  10.4 11.2 19.8 13.3 38.3 38.1 57.4
 VAL-215   ASP-216  7.7 8.4 -2.9 39.2 131.6 156.8 115.4
 ASP-216   LEU-217  7.2 8.8 -110.8 69.7 119.9 117.1 -94.6
 LEU-217   PRO-218  3.7 5.0 19.9 -15.2 64.6 60.5 7.1
 PRO-218   ALA-219  3.1 4.5 24.1 -5.8 120.8 124.8 -20.3
 ALA-219   VAL-220  4.9 6.4 -5.6 -1.7 111.1 87.3 -1.7
 VAL-220   SER-221  4.6 5.0 42.2 -29.1 60.8 70.9 -1.8
 SER-221   GLU-222  3.0 3.0 13.7 -3.4 27.1 29.0 34.2
 GLU-222   LYS-223  4.6 4.6 5.0 -3.3 110.2 101.6 6.9
 LYS-223   ASP-224  7.6 7.9 0.9 -5.1 107.1 101.3 14.4
 ASP-224   ILE-225  7.5 8.0 19.4 -9.4 44.5 35.1 16.0
 ILE-225   GLN-226  8.6 8.4 -5.7 -9.9 101.4 109.7 4.4
 GLN-226   ASP-227  10.7 11.0 20.6 -1.2 129.9 129.8 -29.7
 ASP-227   LEU-228  12.3 12.6 -11.0 0.3 96.7 97.7 -16.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees