Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-86   PRO-87  2.9 3.1 22.8 -11.5 97.9 111.6 -29.2  PRO-87   GLU-88  5.5 5.7 -83.6 59.3 36.1 45.0 87.6  GLU-88   ILE-89  8.0 7.5 -1.4 -6.6 76.6 83.1 10.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-186   PRO-187  9.6 10.0 -3.1 1.6 54.3 63.4 -11.4  PRO-187   ALA-188  8.5 8.5 -16.6 10.0 99.1 104.3 -12.8  ALA-188   VAL-189  5.9 5.6 -2.0 -3.3 61.7 43.0 31.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees