Aspartate Aminotransferase (E.C.2.6.1.1)

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-33   ASN-34  14.3 14.8 4.5 -5.7 132.9 130.4 17.2  ASN-34   LEU-35  12.9 12.8 -7.4 -12.5 70.5 68.0 122.1  LEU-35   GLY-36  10.0 10.0 18.5 18.0 127.7 135.8 -270.4  GLY-36   ILE-37  10.5 10.8 -128.3 23.4 121.4 95.9 -422.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-38   VAL-39  7.8 7.5 106.6 -32.9 38.9 28.2 845.9  VAL-39   TYR-40  7.7 7.4 -14.4 5.6 112.9 116.5 -28.1  TYR-40   LYS-41  4.4 4.4 -0.7 0.5 145.8 140.9 7.7  LYS-41   ASP-42  4.3 4.3 1.9 -11.2 63.8 63.0 -71.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-330   GLN-331  4.2 4.1 -1.5 4.1 119.2 120.5 50.5  GLN-331   ARG-332  7.7 7.6 0.9 5.0 110.4 111.6 -11.1  ARG-332   MET-333  9.3 9.1 -22.1 19.5 110.4 112.1 73.9  MET-333   ARG-334  7.9 7.9 -0.2 4.8 166.9 165.9 27.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-355   GLN-356  10.1 9.6 9.3 -4.3 86.8 90.5 -9.7  GLN-356   ASN-357  6.5 5.9 -7.2 9.1 149.4 146.7 15.4  ASN-357   GLY-358  6.0 5.7 7.2 -0.9 71.5 70.8 25.5  GLY-358   MET-359  3.9 3.8 9.3 -5.1 74.4 65.9 -1.2  MET-359   PHE-360  0.8 1.0 -6.8 0.3 44.8 55.6 18.2  PHE-360   SER-361  1.3 1.8 -18.8 60.2 48.2 50.7 -381.4  SER-361   PHE-362  2.9 4.0 -20.8 3.7 65.3 43.9 153.5  PHE-362   SER-363  5.4 4.8 -19.7 -1.4 98.9 86.9 16.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-385   ARG-386  9.8 9.9 3.7 -6.2 54.0 52.1 -9.4  ARG-386   VAL-387  7.0 7.1 -8.2 10.1 159.9 157.8 -19.0  VAL-387   ASN-388  7.0 7.4 8.5 1.3 52.0 50.7 153.3  ASN-388   VAL-389  5.1 5.6 -9.5 -1.8 128.9 125.6 -93.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees