Pyruvate Kinase, M2 Isozyme

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-114   GLY-115  6.5 6.2 -4.2 -15.4 74.7 75.7 31.1
 GLY-115   PRO-116  3.0 3.0 24.1 -4.2 90.0 103.7 5.0
 PRO-116   GLU-117  4.5 5.2 -78.3 57.1 28.7 34.1 69.9
 GLU-117   ILE-118  7.3 7.0 -3.5 -4.4 95.9 94.5 -10.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-217   PRO-218  9.2 9.2 3.8 -10.9 124.3 113.8 13.9
 PRO-218   ALA-219  7.2 6.9 -6.5 -9.3 117.2 116.1 -7.3
 ALA-219   VAL-220  4.9 4.4 -11.6 9.9 85.8 57.7 9.3
 VAL-220   SER-221  1.8 2.2 -52.3 13.8 72.4 56.2 68.8
 SER-221   GLU-222  4.4 4.5 6.1 2.2 153.6 148.7 -12.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees