Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-713   LYS-714  6.8 6.6 9.9 -1.8 147.9 145.5 -34.4  LYS-714   GLY-715  7.5 7.2 -11.1 -5.2 68.7 72.1 46.3  GLY-715   PRO-716  4.2 4.1 13.4 -15.8 109.5 122.2 22.3  PRO-716   GLU-717  5.4 5.5 -91.3 80.0 28.8 26.1 67.7  GLU-717   ILE-718  7.8 7.0 -2.2 6.6 70.9 70.9 16.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-816   LEU-817  11.3 11.3 -1.6 6.0 56.0 45.3 15.7  LEU-817   PRO-818  9.2 9.4 1.4 -4.6 140.4 134.6 23.9  PRO-818   ALA-819  7.8 7.9 -10.5 -1.9 98.8 99.4 7.1  ALA-819   VAL-820  4.5 4.5 5.8 -7.1 100.2 124.1 4.1  VAL-820   SER-821  3.8 4.0 -29.7 -8.0 90.8 77.2 55.7  SER-821   GLU-822  7.2 7.4 -1.0 7.9 23.9 30.6 -25.6  GLU-822   LYS-823  9.4 9.5 -1.9 1.1 84.9 94.5 -9.6  LYS-823   ASP-824  9.7 9.9 -1.9 -8.8 76.3 81.0 17.8  ASP-824   ILE-825  6.2 6.3 17.1 -12.0 167.4 164.8 -8.6  ILE-825   GLN-826  5.5 5.8 -4.7 9.8 62.7 63.5 -10.0  GLN-826   ASP-827  8.7 9.1 -7.3 7.0 102.2 103.1 -26.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees