Dna-Directed RNA Polymerase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ARG-142   SER-143  5.3 5.2 3.2 -4.9 43.1 44.5 -28.5
 SER-143   PRO-144  3.3 2.9 -21.7 21.0 128.2 131.8 -18.4
 PRO-144   GLY-145  5.2 4.6 16.2 -26.9 102.0 99.9 61.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ILE-163   PRO-164  9.7 9.0 -10.5 19.4 46.7 47.9 -26.8
 PRO-164   LEU-165  7.7 7.1 -2.0 -6.0 82.8 88.7 -15.0
 LEU-165   PRO-166  5.2 5.0 -3.6 0.0 40.3 41.6 103.3
 PRO-166   LYS-167  5.4 5.7 2.3 -12.4 78.6 81.7 -55.4
 LYS-167   ARG-168  9.1 9.4 20.2 -21.2 138.5 143.4 -31.9

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ILE-261   ALA-262  5.5 5.7 21.6 -32.6 44.8 48.8 -171.8
 ALA-262   ASP-263  4.9 4.6 50.8 -22.5 18.6 30.9 505.8
 ASP-263   PRO-264  7.3 7.5 4.8 -4.0 49.0 30.3 -155.0
 PRO-264   ARG-265  7.0 7.4 115.4 104.6 89.5 64.6 -312.0
 ARG-265   ARG-266  3.8 4.6 -47.2 6.6 71.7 146.0 1224.3
 ARG-266   TYR-267  3.4 3.6 123.3 29.2 40.8 120.0 -504.6
 TYR-267   ASP-268  4.2 3.2 164.1 -91.2 96.7 107.1 -127.0
 ASP-268   LEU-269  8.1 7.0 13.5 5.8 87.4 85.7 11.0
 LEU-269   GLY-270  10.3 9.8 7.2 -15.8 51.5 48.7 -130.7
 GLY-270   GLU-271  12.6 12.2 -9.9 2.0 164.7 160.8 -134.8
 GLU-271   ALA-272  11.0 10.6 6.5 -2.4 79.5 76.9 84.3
 ALA-272   GLY-273  7.2 6.8 0.2 1.0 69.8 69.1 -23.8
 GLY-273   ARG-274  7.8 7.7 -3.0 5.0 107.3 101.7 -89.0
 ARG-274   TYR-275  11.6 11.5 -31.2 27.3 157.6 159.0 -74.4
 TYR-275   LYS-276  11.4 11.7 -2.1 4.6 125.1 124.9 27.8
 LYS-276   ALA-277  10.2 10.2 -25.6 15.3 79.1 80.1 64.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ILE-333   ARG-334  4.8 5.4 8.1 -7.2 152.4 155.8 -34.8
 ARG-334   THR-335  6.8 7.2 6.9 1.2 85.7 89.1 -38.4
 THR-335   VAL-336  9.9 10.5 -2.0 -5.7 39.1 42.1 145.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees