Glycosyltransferase Gtfa

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-180   ALA-181  6.0 6.0 -11.0 17.9 74.7 71.6 0.6  ALA-181   ASP-182  3.0 3.1 10.9 -6.4 91.5 85.8 22.4  ASP-182   PRO-183  0.9 0.7 -8.3 3.5 24.4 34.9 14.8  PRO-183   VAL-184  3.2 3.6 5.2 -15.8 123.3 126.1 38.6  VAL-184   LEU-185  5.4 5.2 -6.5 6.5 62.5 61.2 -0.9  LEU-185   SER-186  4.6 4.4 6.4 -5.0 125.6 126.6 -24.4  SER-186   PRO-187  1.7 1.2 1.5 1.4 104.2 100.2 -26.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TRP-201   ILE-202  4.5 4.4 -4.2 3.9 114.0 108.8 4.5  ILE-202   LEU-203  4.7 4.9 -7.2 21.9 177.3 178.9 101.9  LEU-203   PRO-204  5.9 6.3 12.5 -8.8 70.1 72.9 -19.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-370   ILE-371  10.1 10.9 -10.9 2.4 81.3 80.1 54.9  ILE-371   ARG-372  6.9 7.5 16.0 -35.3 137.1 149.8 95.8  ARG-372   ALA-373  4.7 5.6 27.6 -24.2 142.3 123.2 8.0  ALA-373   ASP-374  3.8 3.5 5.8 13.9 118.7 122.7 -58.5  ASP-374   GLY-375  3.5 3.7 -5.6 8.6 117.1 115.5 4.9  GLY-375   THR-376  3.1 3.4 -6.6 0.9 55.6 54.0 17.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees