Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PHE-165   TYR-166  16.7 16.3 4.3 -3.3 74.3 77.6 -0.1
 TYR-166   ARG-167  17.0 16.7 9.2 -21.9 38.8 41.0 -30.1
 ARG-167   ASP-168  14.4 14.1 23.3 -27.6 39.6 45.0 -129.5
 ASP-168   GLN-169  14.5 12.6 0.8 21.6 73.0 81.3 52.2
 GLN-169   PRO-170  10.7 9.6 -47.0 -5.4 30.9 12.1 207.4
 PRO-170   HIS-171  8.4 8.1 5.1 14.1 126.9 121.6 -23.6
 HIS-171   PRO-172  7.4 7.5 -5.5 4.4 108.3 104.0 -17.5
 PRO-172   PHE-173  5.0 5.4 -2.6 -6.4 61.8 63.6 22.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASP-222   ARG-223  8.2 7.8 3.3 14.1 33.3 43.3 72.2
 ARG-223   LYS-224  6.0 5.4 5.2 19.9 34.1 24.7 96.5
 LYS-224   ALA-225  6.1 5.5 -22.0 -27.5 108.0 125.0 -102.0
 ASP-231   ASP-232  10.1 10.9 -98.8 113.7 33.2 30.1 -60.8
 ASP-232   LEU-233  9.9 10.1 4.1 -21.6 100.1 81.8 9.6
 LEU-233   LEU-234  8.7 8.7 -9.2 15.0 102.8 112.5 18.9
 LEU-234   THR-235  4.9 4.9 0.8 -10.1 14.1 9.5 -38.4
 THR-235   HIS-236  5.1 5.6 -0.4 16.7 110.0 118.7 37.5
 HIS-236   MET-237  7.1 7.7 -13.7 -8.1 67.7 89.8 -8.8
 MET-237   LEU-238  5.9 6.2 7.9 15.7 55.8 54.9 12.7
 LEU-238   ASN-239  3.1 3.0 -0.2 19.7 169.6 162.8 82.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees