Ribose Abc Transporter, Periplasmic Ribose- Binding Protein

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLN-101   ILE-102  4.0 4.1 -6.8 5.9 139.5 139.5 39.7  ILE-102   TYR-103  2.9 3.1 0.9 -0.5 68.2 67.2 -5.9  TYR-103   SER-104  1.3 1.0 0.4 0.8 71.4 69.1 12.6  SER-104   ASP-105  3.6 3.4 -11.8 2.9 48.5 48.8 42.4  ASP-105   ASN-106  6.8 6.4 6.7 6.0 80.3 80.8 26.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  MET-243   GLN-244  7.4 7.3 6.2 0.1 40.8 43.4 22.2  GLN-244   PHE-245  4.8 4.4 -2.6 2.0 57.6 56.5 -12.7  PHE-245   PRO-246  1.3 0.7 0.1 -1.1 64.0 62.8 8.1  PRO-246   LYS-247  4.2 3.3 -2.7 0.4 74.2 76.2 -6.6  LYS-247   LEU-248  5.2 5.0 1.5 -3.4 62.1 63.2 -0.3  LEU-248   MET-249  4.5 4.8 12.8 -9.8 20.7 12.0 0.2  MET-249   ALA-250  4.2 4.1 6.7 0.2 83.0 79.8 28.4  ALA-250   ARG-251  7.7 7.4 0.3 -7.6 96.9 96.3 -1.5  ARG-251   LEU-252  9.2 9.3 12.6 -1.5 39.7 43.5 51.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-270   VAL-271  3.9 3.9 -4.1 -9.5 143.7 146.2 34.2  VAL-271   PRO-272  4.8 4.8 -17.9 5.6 118.6 129.4 -17.8  PRO-272   VAL-273  4.3 4.5 -7.5 5.1 165.0 155.5 -25.9  VAL-273   THR-274  3.8 4.3 -2.4 4.7 113.3 118.0 38.0  THR-274   VAL-275  6.5 6.8 10.4 -9.9 52.5 45.7 7.4  VAL-275   GLU-276  7.1 7.2 1.7 1.8 51.9 53.6 27.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees