Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 HIS-171   PRO-172  5.3 4.7 -9.6 22.5 92.6 86.8 -15.5
 PRO-172   PHE-173  4.5 4.1 -10.0 -12.6 61.3 68.4 39.6
 PHE-173   ILE-174  1.6 1.2 -0.5 8.6 128.5 127.1 16.1
 ILE-174   THR-175  0.9 1.5 17.0 -13.0 47.4 44.2 9.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASP-214   LEU-215  6.3 6.1 8.8 -9.5 84.9 87.1 -3.9
 LEU-215   VAL-216  3.2 2.8 11.7 1.3 36.8 39.3 22.8
 VAL-216   ASP-217  1.4 1.7 -8.2 9.7 147.0 142.4 7.9
 ASP-217   LYS-218  4.0 4.0 -0.8 5.2 100.8 100.9 6.1
 LYS-218   ILE-219  5.0 4.7 -25.3 23.0 106.1 113.3 18.1
 ILE-219   ILE-220  3.6 3.4 -5.9 8.3 168.5 169.0 -0.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees